Contamination in Tissue Culture by Jorgen Fogh

By Jorgen Fogh

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Biol 10, 411. , and Ludovici, P. P. (1971). In Vitro 7, 26. Foley, G. , Handler, A. , Lynch, P. , Woman, S. , Stulberg, C. , and Eagle, H. (1965). Cancer Res. 25, 1254. Foley, G. , Uzman, B. , and Adams, R. A. (1968). /«"The Proliferation and Spread of Neoplastic Cells," Univ. , Symp. Monogr. No. 21, p. 65. Williams & Wilkins, Baltimore, Maryland. Ford, D. , and Yerganian G. (1958). J. Nat. Cancer Inst. 21, 393. Franks, D. (1966). Vox Sang. 11, 674. Franks, D. (1967). In Vitro 2, 54. Franks, D.

However, the m e t h o d s were not widely adopted because most workers were unable to avoid loss of cell cultures through contamination with bacteria and fungi. Only those w h o were willing to devise and observe rigid aseptic techniques were successful. , 1945; Early and Morgan, 1946) and the use of replicate 46 Lewis L. , 1951) opened the door for mass cultivation and quantitative measurements in many disciplines of biology (Chang, 1954; D u l b e c c o and Vogt, 1954; Sanford et al, 1948; Bang and Gey, 1951; Coriell al, 1967; Whitefield and Lindell, 1969).

Pathol. 29, 113. Scherer, W. , Syverton, J. , and Gey, G. O. (1953). J. Exp. Med. 97, 695. , Chiu, S. , Buoen, L. , and Brand, K. , (1967). , Allerg. Klin. Immunol. 134, 23. Simpson, W. , and Stulberg, C. S. (1963). Nature (London) 189, 616. Simpson, W. , and Stulberg, C. S. (1971). Unpublished data. Spear, G. S. (1962). Bull. Johns Hopkins Hosp. I l l , 307. Stulberg, C. S. (1968). Nat. , Monogr. 29, 15. Stulberg, C. , Simpson, W. , Peterson, W. (1961a). Fed. Proc, Fed. Amer. Soc. Exp. Biol. ).

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