Cell Culture Techniques in Heart and Vessel Research by A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)

By A. C. Nag (auth.), Prof. Dr. Dr. H. M. Piper (eds.)

In the cardiovascular sciences, an expanding call for for using sleek tools of mobile biology has built. using particular phone tradition versions of many of the tissues concerned is vital for many of those novel methods. This booklet meets the call for for acomprehensive and simple obtainable resource for mobile cul- ture tools in cardiovascular learn because it was once no longer formerly to be had. the fundamental equipment for cultures of cardiomyocytes (embryonic and adult), endothelial cells (micro- and macrovascular), delicate muscle cells and pericytes are defined intimately through a world choice of specialists. particular chapters talk about using progress elements and attachment substrates, concepts for co-cultures, cultures on permeable filter out membranes and microcarrier cultures. The methodological descriptions are sufficiently unique for an instantaneous software within the laboratory. All chapters additionally include a serious assessment of other approaches.

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Temperature-controlled water circulator, for 37°C temperature control of the Langendorff system 7. Pasteur pipette for gassing the top reservoir Instruments - 2 Scissors (coarse and fine) 2 Small forceps 2 Large Petri dishes (200 mm in diameter) 2 Scalpels and a watchglass (or a tissue chopper) Adult Ventricular Rat Heart Muscle Cells 39 r~ser­ voir A B 100 em reser- voir 95",02 5'/, C02 Fig. 1. 4). Prepare 11, warm up, and gasequilibrate before the experiment. - Ca stock solution is 100 mM CaCI 2 in H 2 0.

Frelin C, Padieu P (1976) Pleiotypic response of rat heart cells in culture to serum stimulation. Biochimie 58:953-959 19. Girardi AJ, Warren J, Goldman C, Jeffries B (1958) Growth and CF antigenicity of measles virus in cells deriving from human heart. Proc Soc Exp Bioi Med 98: 18-22 20. Gordon HP, Brice MC (1974) Intrinsic factors influencing the maintenance of contractile embryonic heart cells in vitro. I. The heart muscle conditioned medium effect. Exp Cell Res 85:303-310 21. Gordon HP, Brice MC (1974) Intrinsic factors influencing the maintenance of contractile embryonic heart cells in vitro.

Frelin C (1978) The growth of heart cells in culture. Evidence for a multiple activation of the pleiotypic program. Biochimie 60:627-638 17. Frelin C (1980) The regulation of protein turnover in newborn rat heart cell cultures. J Bioi Chern 255:11149-11155 18. Frelin C, Padieu P (1976) Pleiotypic response of rat heart cells in culture to serum stimulation. Biochimie 58:953-959 19. Girardi AJ, Warren J, Goldman C, Jeffries B (1958) Growth and CF antigenicity of measles virus in cells deriving from human heart.

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