By Marvin J. Weinstein and Gerald H. Wagman (Eds.)
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Extra resources for ANTIBIOTICS: ISOLATION, SEPARATION AND PURIFICATION
The absence of proline was a noteworthy feature of these actinomycins. In a later study32,54y55 4-keto-5-methylproline was identified as a component of Am 2 1 via reduction with aluminum isopropoxide prior to hydrolysis. The formation 0-f two isomers of 4-hydroxy-5methylproline was demonstrated by paper chromatographic comparisons with the synthetic compounds using solvent a. 4-Keto-5methylproline was similarly identified in actinoiiiycin 25 hydro- 20 by PC with solvent lysates, together with 5-methylpr0line~~, which was said to distinguish 3-,4- and 5-methylproline.
3. 1 Introduction................................ 3 . 1 Chemistry. 3 Biological activity ......................... Physical properties ......................... 1 Halomicin A.
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