ANTIBIOTICS: ISOLATION, SEPARATION AND PURIFICATION by Marvin J. Weinstein and Gerald H. Wagman (Eds.)

By Marvin J. Weinstein and Gerald H. Wagman (Eds.)

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Extra resources for ANTIBIOTICS: ISOLATION, SEPARATION AND PURIFICATION

Sample text

The absence of proline was a noteworthy feature of these actinomycins. In a later study32,54y55 4-keto-5-methylproline was identified as a component of Am 2 1 via reduction with aluminum isopropoxide prior to hydrolysis. The formation 0-f two isomers of 4-hydroxy-5methylproline was demonstrated by paper chromatographic comparisons with the synthetic compounds using solvent a. 4-Keto-5methylproline was similarly identified in actinoiiiycin 25 hydro- 20 by PC with solvent lysates, together with 5-methylpr0line~~, which was said to distinguish 3-,4- and 5-methylproline.

3. 1 Introduction................................ 3 . 1 Chemistry. 3 Biological activity ......................... Physical properties ......................... 1 Halomicin A.

Physiol. 7. H . Brockmann a n d H . G r s n e , Chem. Ber. 87 (1954) 1036-1051. 8. Kh. Z e p f , E x p e r i e n t i a , 14 (1958) 207-208. 9. R . B o s s i , R . Hctter, W . K e l l e r - S c h i e r l e i n , L. Neipp a n d H . Zahner, H e l v . Chim. Acta 41 (1958) 1645-1652. 0. H. Brockmann a n d H. G r z n e , N a t u r w i s s . 4 0 (1953) 222-223. 11. L. C. V i n i n g a n d S . A . Waksman, S c i e n c e 120 (1954) 389-3’90. 12. R. Manaker, F. G r e g o r y , L . C . V i n i n g a n d S .

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