Topics in Fluorescence Spectroscopy, Protein Fluorescence by Chris D. Geddes, Joseph R. Lakowicz

By Chris D. Geddes, Joseph R. Lakowicz

Over the decade, fluorescence has turn into the dominant software in biotechnology and clinical imaging. those intriguing advances were underpinned by means of the advances in time-resolved thoughts and instrumentation, probe layout, chemical / biochemical sensing, coupled with our furthered wisdom in biology.

Complementary volumes nine and 10, Advanced options of Fluorescence Sensing: Small Molecule Sensing and complicated innovations of Fluorescence Sensing: Macromolecular Sensing, goal to summarize the present state-of-the-art in fluorescent sensing. therefore, Drs. Geddes and Lakowicz have invited chapters, encompassing a huge diversity of fluorescence sensing thoughts. a few chapters care for small molecule sensors, comparable to for anions, cations, and CO2, whereas others summarize fresh advances in protein-based and macromolecular sensors. The Editors have, besides the fact that, no longer integrated DNA or RNA dependent sensing during this quantity, as this have been reviewed in quantity 7 and is to be the topic of a extra specified quantity within the close to destiny.

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Chem. Soc. 106, 4286–4287 (1984). This page intentionally left blank. 2 Spectral Enhancement of Proteins by in vivo Incorporation of Tryptophan Analogues J. B. Alexander Ross, Elena Rusinova, Linda A. Luck, and Kenneth W. 1. Introduction Tryptophan (Trp) residues in proteins and polypeptides have been used extensively as absorption, fluorescence, and phosphorescence probes for studying structure, dynamics, interactions, and local environments. In particular, changes in fluorescence intensity, emission wavelength maximum, lifetimes, and anisotropy, as well as differential accessibility to quenchers and sensitivity to bound ligands, have made Trp a valuable and widely used spectroscopic tool.

Photobiol. 18, 263–279 (1973). Beechem, J. M. and Brand, L. “Time-resolved fluorescence in proteins” Ann. Rev. Biochem. 54, 43–71 (1985). Longworth, J. W. “Intrinsic Fluorescence of Proteins” in Excited States of Proteins and Nucleic Acids, R. E Steiner and I. Weinryb, eds, Plenum Press, New York, pp. 319–483 (1971). Demchenko, A. P. Ultraviolet Spectroscopy of Proteins, Springer-Verlag, New York (1981). Lakowicz, J. R. Principles of Fluorescence Spectroscopy, New York, Plenum Press (1983). Fluorescence Biomolecules, edited by D.

And Ivkova, M. N. “Fluorescence and the location of tryptophan residues in protein molecules” Photochem. Photobiol. 18, 263–279 (1973). Beechem, J. M. and Brand, L. “Time-resolved fluorescence in proteins” Ann. Rev. Biochem. 54, 43–71 (1985). Longworth, J. W. “Intrinsic Fluorescence of Proteins” in Excited States of Proteins and Nucleic Acids, R. E Steiner and I. Weinryb, eds, Plenum Press, New York, pp. 319–483 (1971). Demchenko, A. P. Ultraviolet Spectroscopy of Proteins, Springer-Verlag, New York (1981).

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